• About
    • About Us
    • What We Cover
  • Advertise
    • Advertise
    • Our Advertisers
  • Contact
  • Donate
  • Send News

Oak Ridge Today

  • Home
  • Sign in
  • News
    • Business
    • Community
    • Education
    • Government
    • Health
    • Police and Fire
    • U.S. Department of Energy
    • Weather
  • Sports
    • High School
    • Middle School
    • Recreation
    • Rowing
    • Youth
  • Entertainment
    • Arts
    • Dancing
    • Movies
    • Music
    • Television
    • Theater
  • Premium Content
  • Obituaries
  • Classifieds

ORNL cell-free protein synthesis is potential lifesaver

Posted at 10:47 am January 3, 2016
By Oak Ridge National Laboratory Leave a Comment

Nanoporous-Membrane-December-2015-ORNL

This section of a serpentine channel reactor shows the parallel reactor and feeder channels separated by a nanoporous membrane. At left is a single nanopore viewed from the side; at right is a diagram of metabolite exchange across the membrane. (Image by ORNL)

 

Lives of soldiers and others injured in remote locations could be saved with a cell-free protein synthesis system developed at the U.S. Department of Energy’s Oak Ridge National Laboratory.

The device, a creation of a team led by Andrea Timm and Scott Retterer of the lab’s Biosciences Division, uses microfabricated bioreactors to help the on-demand production of therapeutic proteins for medicines and biopharmaceuticals. Making these miniature factories cell-free, which eliminates the maintenance of a living system, simplifies the process and lowers cost.

“With this approach, we can produce more protein faster, making our technology ideal for point-of-care use,” Retterer said. “The fact it’s cell-free reduces the infrastructure needed to produce the protein and opens the possibility of creating proteins when and where you need them, bypassing the challenge of keeping the proteins cold during shipment and storage.” [Read more…]

Filed Under: Front Page News, Oak Ridge National Laboratory, U.S. Department of Energy Tagged With: Andrea Timm, bioreactor, Biosciences Division, Carmen Foster, Center for Nanophase Materials Sciences, DOE, DOE Office of Science, electron beam, Funding for this project was provided by the Defense Advanced Research Projects Agency, Leidos, microfabricated bioreactors, Mitchel Doktycz, nanoporous membrane, National Institutes of Health, Northwestern University, Oak Ridge National Laboratory, Peter Shankles, photolithography, Scott Retterer, Small, therapeutic proteins, Towards Microfluidic Reactors for Cell-Free Protein Synthesis at the Point-of-Care, U.S. Department of Energy

ORNL: New tool on horizon for surgeons treating cancer patients

Posted at 8:27 pm June 18, 2015
By Oak Ridge National Laboratory Leave a Comment

Droplet-based Surface Sampling Probe

Oak Ridge National Laboratory’s new droplet-based surface sampling probe speeds the process of analyzing a liver biopsy sample. (Photo courtesy ORNL)

 

Surgeons could know while their patients are still on the operating table if a tissue is cancerous, according to researchers from Oak Ridge National Laboratory and Brigham and Women’s Hospital/Harvard Medical School.

In the journal Analytical and Bioanalytical Chemistry, a team led by ORNL’s Vilmos Kertesz describes an automated droplet-based surface sampling probe that accomplishes in about 10 minutes what now routinely takes 20 to 30 minutes. Kertesz expects that time to be cut to four to five minutes soon. For this proof-of-concept demonstration, researchers rapidly profiled two hormones from human pituitary tissue.

“Instead of having to cut and mount tissue and wait for a trained pathologist to review the sample under a microscope, a technician might soon perform an equally conclusive test in the operating environment,” Kertesz said.

The new mass spectrometry-based technology provides an attractive alternative to the traditional method called immunohistochemistry, or IHC, which looks for specific protein biomarkers to make a diagnosis. Although the IHC approach provides a high degree of spatial recognition, it is time-consuming and limited by the quality and specificity of the antibody used to detect the protein. [Read more…]

Filed Under: Front Page News, Oak Ridge National Laboratory, U.S. Department of Energy Tagged With: Aaron Sharp, AB Sciex, Analytical and Bioanalytical Chemistry, anitbody, biomarker, Brigham and Women’s Hospital/Harvard Medical School, cancer, cancer patients, Daniel E. Ponton Fund of the Neurosciences, DFCI Pediatric Low-Grade Astrocytoma Program, DOE, droplet-based method, Gary Van Berkel, IHC, immunohistochemistry, Nathalie Y.R. Agar, National Institutes of Health, Oak Ridge National Laboratory, Organic and Biological Mass Spectrometry Group, ORNL, pituitary tissue, protein biomarker, sampling probe, surgeon, tumors, U.S. Department of Energy, Vilmos Kertesz

Balajee named director of Cytogenetic Biodosimetry Lab at REAC/TS

Posted at 2:17 pm May 23, 2015
By Oak Ridge Today Staff Leave a Comment

Adayabalam Balajee

Adayabalam Balajee

Research scientist brings more than 20 years of laboratory expertise to the position

Adayabalam Balajee has been named the director of the Cytogenetic Biodosimetry Laboratory, which is operated as part of the Radiation Emergency Assistance Center/Training Site, or REAC/TS, at the Oak Ridge Institute for Science and Education.

In his new position, Balajee will manage the operations and staff at the CBL, which is one of only two labs in the U.S. where chromosome abnormality analysis is used for ionizing radiation dose assessment, a press release said. The process for this analysis starts with a blood sample from someone who a physician thinks may have been exposed to radiation.

Prior to joining the CBL, Balajee held the position of research scientist at the Center for Radiological Research, Department of Radiation Oncology at Columbia University Medical Center in New York, where he had been employed since 1999. Balajee also spent five years as a visiting fellow at the Gerontology Research Center at the National Institutes of Health in Baltimore. [Read more…]

Filed Under: Front Page News, Oak Ridge Associated Universities, U.S. Department of Energy Tagged With: Adayabalam Balajee, Banaras Hindu University, blood sample, CBL, Center for Radiological Research, chromosome abnormality analysis, Columbia University Medical Center, Cytogenetic Biodosimetry Laboratory, cytogenetics, Department of Radiation Genetics and Chemical Mutagenesis, Department of Radiation Oncology, Gerontology Research Center, ionizing radiation dose assessment, molecular biology, National Institutes of Health, National Nuclear Security Administration, NNSA, Oak Ridge Associated Universities, Oak Ridge Institute for Science and Education, Office of Worker Safety and Health, radiation, Radiation Emergency Assistance Center/Training Site, REAC/TS, research scientist, Sylvius Laboratories, U.S. Department of Energy, U.S. Nuclear Regulatory Commission

Supercomputers help ORNL researchers identify molecular switch that controls cell behavior

Posted at 12:39 am December 18, 2013
By Oak Ridge National Laboratory Leave a Comment

E. coli Chemoreceptor Switch

Red and blue molecules represent a conformational switch essential to the signaling mechanism of an E. coli chemoreceptor that researchers discovered using computational molecular dynamics simulations. (Image credit: Davi Ortega)

If scientists can control cellular functions such as movement and development, they can cripple cells and pathogens that are causing disease in the body.

Supported by National Institutes of Health grants, researchers at Oak Ridge National Laboratory, the University of Tennessee, and the UT-ORNL Joint Institute for Computational Sciences discovered a molecular “switch” in a receptor that controls cell behavior using detailed molecular dynamics simulations on a computer called Anton built by D.E. Shaw Research in New York City. To study an even larger signaling complex surrounding the switch, the team is expanding these simulations on Titan—the nation’s most powerful supercomputer, managed by the Oak Ridge Leadership Computing Facility at ORNL.

Researchers identified the molecular switch on Anton (which was designed to perform speedy molecular dynamics simulations) by simulating 140,000 atoms that make up the signaling part of the Tsr chemoreceptor that controls motility in E. coli. Like other receptors, Tsr spans the cell membrane, communicating to proteins inside the cell in order to respond to threats or opportunities in the environment. [Read more…]

Filed Under: Front Page News, Oak Ridge National Laboratory, Science Tagged With: Anton, cell behavior, chemoreceptor, D.E. Shaw Research, Davi Ortega, dimer, E. coli, Igor Zhulin, Jerome Baudry, JICS, John Parkinson, molecular switch, National Institutes of Health, Nature Communications, Oak Ridge Leadership Computing Facility, Oak Ridge National Laboratory, ORNL, ORNL Computer Science and Mathematics Division, Phe396, phenylalanine amino acids, phenylalanine pairs, receptor, signaling, supercomputer, Titan, Tsr, University of Tennessee, University of Utah, UT Biochemistry and Cellular and Molecular Biology Department, UT Department of Microbiology, UT-ORNL Center for Molecular Biophysics, UT-ORNL Joint Institute for Computational Sciences

Bioluminescence technology from UT a top innovation, researchers have ORNL connection

Posted at 11:27 am December 11, 2013
By University of Tennessee Leave a Comment

University of Tennessee Bioluminescence and Microtiter Plate

Pictured above is a micro titer plate showing light signals in cells, illustrating bioluminescence technology. (Photo courtesy University of Tennessee)

KNOXVILLE—Technology that lights up cells to enable study of the effects of drugs and monitor disease is among The Scientist magazine’s Top 10 innovations of 2013. It’s also a University of Tennessee technology and licensed by a Knoxville-based startup company.

Technology using humanized bacterial luciferase developed by UT researchers and licensed by 490 BioTech, founded by two UT Knoxville faculty members and two then-graduate assistants, is ranked sixth on the magazine’s list. For more about the innovation list, visit here.

“The development of this technology originated more than 10 years ago, and with hard work by past graduate students and key financial support from the National Institutes of Health, the National Science Foundation, and University of Tennessee Research Foundation, it evolved into a marketable product that can be used to make pharmaceutical drugs safer, better, and cheaper,” said Steven Ripp, co-founder and chief operating officer of 490 BioTech and research associate professor at UT Knoxville. “Being recognized as a Top 10 technology is a significant milestone for our company, and it will greatly assist in mainstreaming our product toward better disease management and improved health care.” [Read more…]

Filed Under: College, Education, Front Page News, Oak Ridge National Laboratory, Science Tagged With: 490 BioTech, bioluminescence, bioluminescent tests, Dan Close, David Washburn, Gary Sayler, humanized bacterial luciferase, light signal, National Institutes of Health, National Science Foundation, Oak Ridge National Laboratory, ORNL, Stacey Patterson, Steven Ripp, The Scientist, University of Tennessee, University of Tennessee Research Foundation, UT, UT Center for Environmental Biotechnology, UT-ORNL Joint Institute for Biological Sciences, UTRF

Search Oak Ridge Today

Classifieds

Availability of the draft environmental assessment for off-site depleted uranium manufacturing (DOE/EA-2252)

The U.S. Department of Energy (DOE) National Nuclear Security Administration (NNSA) announces the … [Read More...]

Public Notice: NNSA announces no significant impact of Y-12 Development Organization operations at Horizon Center

AVAILABILITY OF THE FINAL ENVIRONMENTAL ASSESSMENT FOR THE OFFSITE HOUSING OF THE Y-12 DEVELOPMENT … [Read More...]

ADFAC seeks contractors for five homes

Aid to Distressed Families of Appalachian Counties (ADFAC) is a non-profit community based agency, … [Read More...]

Recent Posts

  • Flatwater Tales Storytelling Festival Announces 2025 Storytellers
  • Laser-Engraved Bricks Will Line Walkway of New Chamber Headquarters
  • Democratic Women’s Club to Discuss Climate Change, Energy and Policy
  • Estate Jewelry Show at Karen’s Jewelers Features Celebrity Jewelry
  • Keri Cagle named new ORAU senior vice president and ORISE director
  • ORAU Annual Giving Campaign exceeds $100,000 goal+ORAU Annual Giving Campaign exceeds $100,000 goal More than $1 million raised in past 10 years benefits United Way and Community Shares Oak Ridge, Tenn. —ORAU exceeded its goal of raising $100,000 in donations as part of its internal annual giving campaign that benefits the United Way and Community Shares nonprofit organizations. ORAU has raised more than $1 million over the past 10 years through this campaign. A total of $126,839 was pledged during the 2024 ORAU Annual Giving Campaign. Employees donate via payroll deduction and could earmark their donation for United Way, Community Shares or both. “ORAU has remained a strong pillar in the community for more than 75 years, and we encourage our employees to consider participating in our annual giving campaign each year to help our less fortunate neighbors in need,” said ORAU President and CEO Andy Page. “Each one of our employees has the power to positively impact the lives of those who need help in the communities where we do business across the country and demonstrate the ORAU way – taking care of each other.” ORAU, a 501(c)(3) nonprofit corporation, provides science, health and workforce solutions that address national priorities and serve the public interest. Through our specialized teams of experts and access to a consortium of more than 150 major Ph.D.-granting institutions, ORAU works with federal, state, local and commercial customers to provide innovative scientific and technical solutions and help advance their missions. ORAU manages the Oak Ridge Institute for Science and Education (ORISE) for the U.S. Department of Energy (DOE). Learn more about ORAU at www.orau.org. Learn more about ORAU at www.orau.org. Like us on Facebook: https://www.facebook.com/OakRidgeAssociatedUniversities Follow us on X (formerly Twitter): https://twitter.com/orau Follow us on LinkedIn: https://www.linkedin.com/company/orau ###
  • Children’s Museum Gala Celebrates the Rainforest
  • Jim Sears joins ORAU as senior vice president
  • Oak Ridge Housing Authority Receives Funding Assistance of up to $51.8 Million For Renovating Public Housing and Building New Workforce Housing
  • Two fires reported early Friday

Recent Comments

  • Raymond Mitchell on City manager’s ‘State of the City’ canceled due to weather
  • Raymond Mitchell on City manager’s ‘State of the City’ canceled due to weather
  • Mysti M Desilva on Crews clearing roads, repairing water line breaks
  • Mel Schuster on Crews clearing roads, repairing water line breaks
  • Cecil King on Crews clearing roads, repairing water line breaks
  • Rick Morrow on Roads, schools, businesses closed after heavy snow
  • Diana lively on Free community Thanksgiving Dinner on Nov. 25
  • Anne Garcia on School bus driver arrested following alleged assault on elementary student
  • Raymond Dickover on Blockhouse Valley Recycling Center now open 6 days per week
  • Mike Mahathy on School bus driver arrested following alleged assault on elementary student

Copyright © 2025 Oak Ridge Today